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Crossover junction endodeoxyribonuclease RuvC; Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform framework in supercoiled DNA by nicking to strands with the similar polarity at web-sites symmetrically opposed in the junction while in the homologous arms and leaves a 5'-terminal phosphate plus a three'-terminal hydroxyl group.

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RuvAB can be a helicase that mediates the Holliday junction migration by localized denaturation and reannealing.

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